**Mouse Carbonic Anhydrase 2 (CA-2) ELISA Kit – Instructions for Use**
**Kit Specifications:**
This ELISA kit is available in either a 48-well or 96-well configuration. It is designed for the quantitative determination of Mouse Carbonic Anhydrase 2 (CA-2) in biological samples such as serum, plasma, or cell culture supernatants.
**Standard Dilution:**
The standard reagent is provided in a concentration of 1.5 mL per bottle. The enzyme standard reagent is supplied in 3 mL × 1 bottle (for 48-well) or 6 mL × 1 bottle (for 96-well). Store at 2–8°C.
**Usage and Procedure:**
This reagent is intended solely for research use. To determine the concentration of CA-2 in your samples:
1. Prepare a standard curve by plotting the concentration of the standard on the x-axis and the corresponding OD values on the y-axis using graph paper.
2. Determine the concentration of each sample based on its OD value from the standard curve.
3. Multiply the calculated concentration by the dilution factor to obtain the actual sample concentration.
Alternatively, calculate the linear regression equation of the standard curve using the standard concentrations and their corresponding OD values. Then, substitute the sample OD into the equation to determine the concentration, and multiply by the dilution factor for the actual result.
**Kit Components:**
- Sealing Film: 2 pieces (48-well) / 2 pieces (96-well)
- Standard: 0.5 mL × 1 bottle (2700 ng/L)
- Enzyme Standard: 1×48 / 1×96
- Sample Diluent: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well)
- Developer A: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well)
- Chromogen B: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well)
- Stop Solution: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well)
- Concentrated Wash Solution: (20 mL × 20 times) × 1 bottle (for 48-well) or (20 mL × 30 times) × 1 bottle (for 96-well)
**Storage Conditions:**
All components should be stored at 2–8°C, except for the Chromogen B solution, which should be stored at -20°C. Ensure all reagents are protected from light and contamination during storage.
**Notes:**
Always follow the manufacturer's instructions carefully. Proper handling and accurate measurement are essential for reliable results. This kit is not suitable for diagnostic or clinical use.
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