**Mouse Carbonic Anhydrase 2 (CA-2) ELISA Kit – Instructions for Use**
**Kit Specifications:**
This Mouse Carbonic Anhydrase 2 (CA-2) ELISA Kit is available in a 48-well or 96-well format. It is designed for quantitative detection of CA-2 in biological samples such as serum, plasma, or cell culture supernatants. The kit is intended for research use only and not for diagnostic or clinical purposes.
**Standard Dilution:**
- Enzyme Standard Reagent: 1.5 mL × 1 bottle
- For 48-well format: 3 mL × 1 bottle
- For 96-well format: 6 mL × 1 bottle
**Standard Curve Preparation:**
To determine the concentration of CA-2 in your samples, prepare a standard curve by plotting the standard concentrations on the x-axis and the corresponding OD values on the y-axis using graph paper. Alternatively, calculate the linear regression equation from the standard curve data and use it to determine the sample concentration based on their OD readings. Multiply the calculated value by the dilution factor to obtain the actual concentration of the sample in the original specimen.
**Kit Components:**
- Sealing Film: 2 pieces (for 48-well) / 2 pieces (for 96-well)
- Standard: 0.5 mL × 1 bottle, 2700 ng/L
- Enzyme Standard: 1×48 / 1×96 (store at 2–8°C)
- Sample Diluent: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well) (store at 2–8°C)
- Developer A: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well) (store at 2–8°C)
- Chromogen B Solution: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well) (store at -8°C)
- Stop Solution: 3 mL × 1 bottle (48-well) / 6 mL × 1 bottle (96-well) (store at 2–8°C)
- Concentrated Wash Solution: 20 mL × 20 times (for 48-well) / 20 mL × 30 times (for 96-well) (store at 2–8°C)
**Storage Conditions:**
All reagents should be stored at 2–8°C unless otherwise specified. Chromogen B solution must be kept at -8°C to maintain stability.
**Preparation and Usage Notes:**
Before starting the assay, ensure all reagents are brought to room temperature. Carefully follow the step-by-step protocol provided in the manual. Avoid cross-contamination by using separate pipette tips for each step. Proper washing is essential for accurate results. Always read the absorbance at 450 nm using a microplate reader.
**Important:** This kit is not intended for human diagnostic use. Results should be interpreted with caution and validated with additional methods if necessary.
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