Our unit is planning to purchase a GPC purification system, and we have several questions that need clarification:
1. Is it necessary to include a UV detector with the GPC system? If we are conducting research, does it make sense to pair it with a detector?
2. I've heard that GPC can be quite expensive. Does this refer to solvent costs, or do we need to use chromatography-grade solvents? Are imported solvents required for optimal performance?
3. The single-sample processing time seems long—using a fast column, it takes about 50 minutes (including pre-concentration, GPC, and quantitative concentration). What's the typical solvent consumption for one sample?
A: 1. It's highly recommended to include a UV detector, especially for scientific research. Without one, it's very challenging to analyze new substances or develop new methods. We previously purchased a GPC system without a detector, which made the process very cumbersome. All parameters were fixed, and any changes made it difficult to predict when peaks would appear.
2. GPC is a purification technique, so the quality of reagents is important. While not necessarily imported, they must meet high standards for your experiments. Solvent usage tends to be significant, so you should plan accordingly.
3. The typical flow rate is around 5 ml/min, and the actual purification time ranges from 20 to 40 minutes. When adding in extraction, concentration, and cleaning steps, the total volume processed per sample can be between 400 to 500 ml. This makes solvent consumption an important factor to consider in terms of cost and logistics.
Overall, while GPC is a powerful tool for sample preparation, it's important to evaluate your specific needs before purchasing. A detector can greatly enhance your ability to monitor and optimize the purification process, and solvent choice and usage should be factored into your budget and workflow planning.
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