Our unit is planning to purchase a GPC purification system, and there are several questions we need to clarify:
1. Is it necessary to include a UV detector with the GPC system? If we are conducting research, would it be essential to have one?
2. I've heard that GPC can be quite expensive. Does this refer to the cost of solvents, or is it due to the need for high-purity solvents that must be chromatographically grade?
3. The time required for processing a single sample seems quite long—using a fast column, it still takes about 50 minutes (including pre-concentration, GPC, and quantitative concentration). What is the typical solvent consumption during this process?
A: 1. It's highly recommended to include a UV detector, especially if you're doing scientific research. Without a detector, it's very challenging to analyze new compounds or develop new methods. We previously purchased a GPC system without a detector, and it was extremely inconvenient to use. All parameters were fixed, and any changes made it difficult to predict when the peak would appear.
2. GPC is a purification technique, so the quality of the reagents used is important. While not necessarily imported, they should meet high standards to ensure reliable results. Solvent consumption tends to be relatively high, which is a factor in the overall cost.
3. At a typical flow rate of 5 mL/min, the purification process itself usually takes between 20 to 40 minutes. When you add in the steps like extraction, concentration, and cleaning, the total volume used per sample can range from 400 to 500 mL. This makes solvent usage an important consideration when planning experiments and budgeting.
Additionally, it's worth noting that the efficiency of the GPC system can also depend on the type of samples being processed. For complex mixtures, more time and solvent may be needed to achieve the desired level of purity. It’s also advisable to consider the maintenance and operational costs, as these can add up over time. Investing in a system with a detector and good solvent management features could save both time and resources in the long run.
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