The semi-solid medium contains 0,02% -0.3% agar, which is enough to prevent the flow of the medium, thereby preventing the oxygen from dissolving into the medium.
One of the most suitable semi-solid medium is to add 0.1% sodium thioglycolate and 0.02% -0.3% agar to nutrient medium (such as tryptone broth medium or nutrient broth medium) 〖Prevent air flow〗 , And then aliquot it into MacConkey bottles or test tubes. Add 0.0002% methylene blue (containing 0,2ml, 1% methylene blue solution per liter of medium) as a redox potential indicator
Add these substances in the nutrient broth, mix well. After heating and dissolving, dispense 12ml of each tube into 150mm * 16mm test tubes, stopper tightly and sterilize at 121 ℃ for 15min. Methylene blue is used as a redox indicator. The oxidation reaction makes the medium blue, and the medium is colorless after the reduction reaction. This medium will become blue-green after storage, and it should be heated in a boiling water bath for 10 min before use to remove oxygen.
Fortified Clostridium (RCM)
This medium was developed in 1954. Although it is not suitable for the growth of all anaerobic bacteria, it is still useful for counting many anaerobic bacteria, such as anaerobic bacteria in food samples. Containing glucose and cysteine ​​as reducing substances, this medium is divided into 25ml Macconkey bottles with screw caps, without the need to seal with paraffin or other substances, it can provide a growth environment for anaerobic bacteria . In the detection of anaerobic bacteria live cell count, it is recommended to use only as a diluent.
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