The working principle and usage principle of the constant Ord upgraded microbial sampler
The six-stage air microbial sampler is a six-stage mesh impingement air microbial sampler. It is a dual-function stepped multi-stage impact sampler that can be widely used in sanitation, bio-cleaning, pharmaceutical, fermentation, and other environments. The monitoring and related research and teaching departments conduct sampling research on airborne microorganisms, and the air microbial sampler (level 6) provides a basis for evaluating the microbial pollution hazards and their prevention measures in the air environment.
working principle
In addition to being able to measure the number of airborne microorganisms, the six-stage mesh-impact air microbial sampler measures the size of these particles, which is one of the important indicators for determining the microbial hazard of air. It is composed of six impactors, each stage is actually a single-stage sampler. With 6 repeated impact principles, most of the particles, especially in the trachea and lung sedimentation particles, basically collide, so it collects The range of particle sizes is naturally wider than that of a single stage, which is unmatched by some single-stage impact samplers, and the round nozzle of the sampler has higher sampling efficiency than the nozzles such as cracks. The relative humidity increased step by step (from 39% in the first stage to 88% in the sixth stage), which is very beneficial for the survival of vulnerable pathogenic microorganisms, especially virions. Because of its distinctive characteristics, it has been widely and effectively applied to the monitoring of airborne microorganisms, and it has not been used since its inception. Recommended by experts as an international standard sampler. The six-stage air microbial sampler simulates the anatomical structure and aerodynamic physiological characteristics of the human respiratory tract. The principle of inertial impact is used to collect the microbial particles suspended in the air hierarchically on the surface of the sampling medium, and then for culture and microbiological analysis.
Component
The six-stage air microbial sampler consists of an impactor, a main unit (flow meter), a timer, and a tripod.
Principle of use
The impactor is a 6-layer aluminum alloy disc with tiny perforations. The disc was lowered into agar plates with a sealing rubber ring between each disc, and the discs were firmly joined by three spring hooks. Each disc has 400 rows arranged in a circle, reduced layer by layer, and the standard sampling flow rate is 1 cubic å‘Ž (28.3 L/min). When the gas stream containing the microbial particles enters the sampling port of the uppermost layer, due to the layer-by-layer increase of the gas flow, different sizes of microbial ions impinge on the corresponding agar surface according to aerodynamic characteristics. The range of particle sizes captured at each stage is determined by the gas flow velocity of the pores of that stage and the particle interception rate of the previous stage. The particles captured by the upper and lower respiratory tracts of the first and second grades, the particles captured by the lower respiratory tract of the 3rd to 6th grades, replicate the penetration and deposition of these particles in the respiratory tract to a considerable extent. The six-stage air microbial sampler provides a plate with normal nutrient agar.
Latest upgrade features
The serious damage caused by airborne microbial pollution has been paid more and more attention, so the need for sampling and monitoring various air pollution
The demand is even more urgent, and the number and size distribution of airborne microorganisms are two indispensable indicators for evaluating the damage. Our factory produces H22258
In addition to the number of airborne microorganisms, the six-stage mesh percussive air microbial sampler is unique in its ability to measure the size of these particles.
The latter is one of the important indicators for determining the microbial hazard of air. It is a combination of six impactors, each of which is actually a single-stage sampler.
Using the principle of six repeated impacts, most of the particles, especially those in the trachea and lung sedimentation, basically collide, so the size of the particles it collects
The range is naturally wider than a single stage, which is unmatched by some single-stage impact samplers. The circular nozzle of the impactor has a higher sampling than the slit type nozzle
effectiveness. The relative humidity is increased step by step (from 39% in the first stage to 88% in the sixth level), which is very beneficial to vulnerable pathogenic microorganisms.
The survival of virions is not always used since the advent of the world.
use
H22258 six-stage mesh percussive air microbial sampler can be widely used in disease prevention and control, environmental protection, pharmaceutical, fermentation industry,
Sampling and monitoring of the quantity and size distribution of airborne microorganisms in the food industry, biological cleansing, etc., as well as air micro-related research and teaching departments
Biological sampling research provides a scientific basis for evaluating the hazards of environmental air microbial contamination and its treatment measures.
Simulating the anatomical structure and aerodynamic characteristics of the human respiratory tract, using the principle of conventional impact, the microbial particles suspended in the air are collected on the surface of the sampling medium according to the size, and then co-cultured and further microbiological analysis is performed. Characteristics of the number and size distribution of airborne microbial particles.
structure
The whole set of instruments consists of a six-stage impactor, a main unit (flow meter), a timer, and a tripod.
- The impactor consists of six grade aluminum alloy discs with a smiling spray hole. A plate containing the sample medium is placed under the disc, and the six-stage discs are tightly joined together by three spring hooks. Each of the discs is arranged in a circular array of 400 precisely sized orifices. When the air containing the microbial particles enters the sampling port, the gas flow rate is increased step by step, and the microbial particles of different sizes impinge on the surface of the responsive sampling medium according to the aerodynamic characteristics. The first and second grades are similar to those captured by the upper respiratory tract of the human body. The third-sixth grade is similar to the particles captured by the lower respiratory tract, which simulates the penetration and deposition of these particles in the respiratory tract to a considerable extent.
Instructions
(1) Flow correction of the sampler
The H22258 six-stage sieve percussive air microbial sampler is 28.3 L/min, and the flow rate is corrected before sampling.
1. It is necessary to ensure that the holes in the disc are unobstructed, and then assemble the impactor in order, one hand holding the impactor from the upper part and the other hand hanging on the three spring hooks.
2. Connect the impactor outlet to the main engine inlet with a rubber tube and remove the upper cover of the impeller inlet.
3. Plug the main unit into the power supply (AC220V), press the “power switch†on the main unit, and adjust the “flow adjustment†knob to stabilize the flowmeter rotor at 28.3L/min.
Impactor cleaning and disinfection
1. Wash the impactor with warm water with a neutral detergent. It is better to wash it with ultrasonic waves. The plug of the nozzle can be removed.
2. If the orifice is blocked, use high pressure airflow or equipped with a fine needle to remove
3. The six-stage impactor is disinfected with 70% alcohol. Preparation of sampling plates
1. General aerobic air microbial sampling using ordinary agar medium (medium 1.8%-2.0%) If special microorganisms (such as high-nutrient diseases, viruses, fungi, etc.) are collected, the corresponding sampling medium can be used.
2. The plate is made of domestic Φ90 x 18mm glass culture dish and sterilized by high pressure steam for use.
3. Under sterile conditions, pour the agar into the plate by 24-30 ml. The surface of the agar is level with the high-density ring (8 mm) to ensure the best impact distance of 2-3 mm between the nozzle and the agar surface during sampling.
4. Insert the plate into the sampling medium, invert and place in a 37 ° C incubator for 24 hours, no bacteria growth can be used
(4) On-site sampling
1. Open and lock the tripod, adjust the top of the tripod to the horizontal position, place the striker on the disc of the tripod, place the main unit on the table or on the ground, and connect the impactor outlet with a rubber tube → main engine intake mouth.
2. Place the sampling plate in sequence, open the plate cover with one hand, and quickly cover the impact plate with the other hand, then press the upper part of the striker and hang up the three spring hooks.
When placing and removing the sampling plate, a mask must be worn to prevent the bacteria from contaminating the plate.
3. Open the upper cover of the impactor inlet and leave the sampling point 2 meters away to start sampling. The timer can be used to set the sampling time. Refer to the timer instruction manual.
4. The sampling time depends on the degree of pollution of the air environment, but it is better not to exceed 30 minutes. A long-term airflow impact causes the sampling medium to dehydrate and affect the growth of microorganisms.
5. In order to maintain the accuracy of colony counting, it is advisable for each plate to fall below 250. Generally, the outdoor air environment takes 10 minutes, and the indoor air environment takes 1-5 minutes.
6. After sampling, take out the sampling plate and close the lid, pay attention to the order and number, don't make a mistake.
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