Shanghai Hengyuan talks about Elisa qualitative quantification for experimental novice customers

An Elisa experiment novice customer contacted Shanghai Hengyuan He manager to order a box of 96T human CSF kit. In the process of communication and understanding, the customer questions the qualitative and quantitative Elisa. Shanghai Hengyuan Biological Company has a very complete pre-sales and after-sales service and provides experimental technical guidance. Shanghai Hengyuan talked about Elisa qualitative quantification for experimental novice customers:

Elisa is an experimental method for detecting samples by utilizing the specific binding property between antigen and antibody. The color depth of the substrate indicates the content of the sample in the sample. 1. Qualitative Elisa can only roughly indicate that the sample analyte content is above or below a certain value. Qualitative Elisa usually sets a positive control (P) and a negative control (N). The results are indicated by “negative” and “positive” respectively. . The criteria for the determination of "negative" and "positive" in the qualitative determination of Elisa are the positive judgment values ​​determined by the kit, that is, the cut-off value. 2. Quantification Elisa is to make a standard curve through a series of OD values ​​corresponding to different known concentrations of standards, and then substitute the OD value of the sample into the standard curve to calculate the content of the sample in the sample.

Shanghai Hengyuan Biotechnology Co., Ltd. has long been engaged in the import of Elisa kits, biological reagents, antibodies, standards, culture media and other scientific research reagents. Affordable, off-the-shelf, quality guaranteed. Customers can order by phone, fax, E-mail, or online!

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