purpose
To confirm that the ACQUITY UPLC® I-Class system has low cross-contamination performance for a variety of samples (including extremely high concentration samples).
background
When it is necessary to quantify both high and low concentration components in the same chromatographic separation, the biggest challenge is to solve the problem of sample residue. Generally, in order to observe low levels of impurities related to the main analyte, a high concentration of sample must be injected. In order to accurately analyze the impurities in the analyte, the sample residual problem of the analyte must be resolved so that the amount of impurities present in the sample will not be underestimated, thereby affecting the calculated value of impurities. In the analysis of impurities, the sample concentration needs to be very high, and the solution can be quite challenging. Attention should be paid to the components of the diluent, mobile phase, and cleaning solvent, so that the residual amount of the sample is low. System design also plays an important role in solving the problem of sample residue. In general, the simpler the way the sample is introduced into the analysis system, the easier it is to solve the problem of sample residue, especially when the injection method is used to introduce a variety of compounds with large differences in hydrophobicity and polarity.
The ACQUITY UPLC I-Class system can easily solve the challenging cross-contamination problem in the analysis of some related compounds.
solution
The ACQUITY UPLC I-Class system is designed to achieve low cross-contamination and therefore has excellent performance when used to analyze a variety of related compounds. Sample Manager's flow-through needle sampling (FTN) design provides optimized high-precision injections and excellent sample recovery. The gradient solution was used to clean the inside of the needle during the isocratic run, and the outside of the needle during the chromatographic run.
This solves the problem of sample residue well and does not increase the total injection cycle time. In order to confirm that the ACQUITY UPLC I-Class system can easily solve the problem of sample residue, three different compounds with large differences in properties were selected. Chlorine has been viscous and is usually very difficult to remove completely from the injector. Dioctyl phthalate is a very hydrophobic compound and requires a high concentration of acetonitrile to elute it from the column. Caffeine is a hydrophilic substance and is usually not difficult to remove from the injector, making it an excellent probe compound to determine that no sample remains in the system. Each compound was injected at a concentration with a UV response of 1.5 AU ± 0.1 AU, and the residual amount of the sample in the subsequent blank injection was determined. As shown in Figure 1, for all
For these compounds, no measurable residual sample was detected. To determine the residual amount of sample for each compound, a high-concentration sample (20x to 40x concentration) was prepared and injected into ACQUITY UPLC I-Class FTN. The residual sample amount of each compound in the first blank injection was measured, and it was found that the residual sample amounts of the three analytes with large differences in properties were less than 0.001%.
summary
The ability to solve the problem of sample residue for multiple analytes is an important performance of an analytical system. Regardless of the nature of the analyte, the ACQUITY UPLC I-Class system is designed to produce extremely low cross-contamination. Sample Manager's FTN injection platform is designed to be simple and flexible, so the method can be optimized simply and intuitively; therefore, it can meet the requirements of more challenging applications, such as the analysis of low-level related compounds.
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