The use of the membrane method for microbial detection is an internationally recognized standard test method for microbes, which has been recognized by many countries and organizations and is widely used in environmental monitoring, food and beverage industry, cosmetics, pharmaceutical industry quality control, and electronics industries. The filter membrane method has been successfully applied for the detection of microorganisms for many years. It is practical and convenient. It simplifies the microorganism detection procedure. The following describes the method steps and features.
1. The steps of the membrane filtration method
Put the filter membrane with appropriate pore size into the filter, filter the sample, and keep the microorganisms on the surface of the membrane due to the action of the filter membrane. The microbial growth inhibitor in the sample can be removed by rinsing the filter with sterile water after filtration. Then, the filter is placed on the culture medium, and nutrients and metabolites are exchanged through the pores of the filter. The colonies cultivated on the surface of the filter can be counted and related to the sample amount.
2. Test equipment
This method can use stainless steel single, triple (six) without filter, can be autoclaved; can filter a single sample or filter multiple samples at the same time, will save a lot of time when the sample detection amount is large.
Three, the advantages of the membrane method:
1. Compared with the direct method, a large number of samples can be detected.
2. The concentration effect improves the accuracy of microorganism detection.
3. The filter membrane with colonies can be archived as a permanent record of detection.
4. The visible colonies correspond directly to the sample size, and a quantitative result is obtained.
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