High performance liquid chromatography has four important functions.
â‘ Separation and analysis of the component content of the drug: Since a drug often contains multiple components, the chromatographic method is a method that can be separated and quantified. Such as fat-soluble vitamin tablets, containing chromium vitamins, C18 column and methanol containing 1% ammonium carbonate as mobile phase. The vitamin B6, D1, A, E can be separated and the content can be determined at one time. Another example is analgesics or antipyretics. This method can also be used to separate and measure the content of each component.
â‘¡ Intermediate control in drug production: When producing antibiotics, we must first understand the existence of the main body and by-products in the fermentation broth. For example: the fermentation products of penicillin, tetracycline, cephalosporin, erythromycin, daunorubicin, gentamicin, etc., can be analyzed by C18 column and methanol and buffer mobile phase or by ion pair method to analyze the main body And the content of its products. For procaine amide, theophylline, etc., the main content can be determined, and for nitroglycerin, the main content and its degradation products can be determined to ensure the heat of the drug.
â‘¢ Analysis of the residual amount of the drug in the body: This is one of the main contents of the recent research on the efficacy of new drugs. For example, oridonin is a component of plants, which has anti-cancer effects, but it is highly toxic. It is very important to determine its content in serum. Now you can use the C18 column and methanol water as the mobile phase to determine the serum content after treatment to obtain the blood content. If the content of sulfonamides in serum and urine is not allowed to be too large, C18 column and acidic ethanol can be used as the mobile phase. After determining the content, the most appropriate dosage can be estimated. Another example is tetracycline, barbituric acid, analgesics, antipyretics, diabetes drugs, etc., the content in blood or urine can be measured using C18 column with methanol and water as mobile phases. Therefore, the application of reversed-phase chromatography is extremely wide.
â‘£ Determine the metabolites of drugs in various organs, especially study the existence of metabolites of drugs in serum. Such as measuring keto acid and glutaric acid in serum. The measurement of metabolites in urine is more convenient and does not require complicated pretreatment. Because studying the components in urine can identify the metabolic function of patients, it has important reference value for pathological research or bed diagnosis.
Single Beach Chair
with excellent UV resistant PE rattan weaving and alu frame, waterproof polyester or olefin fabric, single beach chair is best choice for your hotel or swimming pool. With a umbrella and a coffee or tea, you can relax in the sandbeach. engoy the sunshine with your friend and family.
Handwoven premium resin wicker UV resistant
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Versatile tempered glass tables
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Single beach chair with different style and hand weaving
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