Hengaode instrument analysis how to buy and use pipette
Pipettes (pipettes) generally use pipettes to measure a small amount or a small amount of liquid when conducting research on analysis and testing. Many people will ignore the correct use of the pipette and some details of its operation.
Pipette (pipette gun) purchase
1. Product performance, that is, the accuracy and repeatability of the pipette. For most users, it is difficult and unnecessary to check product performance before purchasing. Therefore, it is mainly based on the technical data provided by the manufacturer. But here are two points to make clear: first, do n’t easily believe the seller ’s verbal commitment, and you must check the written materials provided by the manufacturer; second, the brands that have a greater impact on the global pipette market, such as the American RAININ Labnet, SOCOREX, EPPENDORF and GILSON, etc., provide higher reliability of technical data.
2. The reliability and durability of the product mainly depends on the material used for the pipette. For the housing, it should have higher impact resistance, corrosion resistance and lower thermal conductivity (such as PVDF material); for the piston, there are mainly three materials on the market at present: stainless steel, ceramic and plastic. Stainless steel has good mechanical properties and long life, but it is not suitable for pipetting of strong acids and bases; ceramics have high corrosion resistance, but poor mechanical properties. Of course, high-quality materials often mean higher prices, so it is necessary to comprehensively consider the factors of purchase price and life.
3. The ergonomic design of the product can mainly consider the following points:
First, the short moving distance of the thumb to complete a pipetting cycle means greater comfort;
Second, compare the force of the thumb required by a pipette of the same range to complete a liquid discharge (be sure to press it all the way down), which is the key to comfort. The less force, the less risk of finger damage caused by long-term use;
Third, loading and unloading the suction head, the same is the less effort the better;
Fourth, the weight of the pipette is moderate, too heavy will increase the burden on the hand, but too light also often means that the material may be unsatisfactory;
Fifth, other auxiliary designs, such as the matte design of the shell and the design of the finger hook, help to further improve comfort. In terms of ergonomics, RAININ is in a leading position in the global pipette market, and has a perfect performance in the above points.
Sixth, the new laboratory equipment, the new force, the innopette pipette of the beyonlab group strictly follows ergonomics, based on the hand data of Europe, America and Asia, and re-made a pipette that is suitable for different regions. further.
Use of pipettes (pipettes)
Range adjustment
When adjusting the range, if you want to change from a large volume to a small volume, you can rotate the knob counterclockwise according to the normal adjustment method; but if you want to adjust from a small volume to a large volume, you can turn the scale knob clockwise to The scale of the range, and then call back to the set volume, so as to ensure the highest accuracy of the measurement.
During this process, never push the button out of the range, otherwise it will jam the internal mechanical device and damage the pipette.
Assembly of the pipette tip (suction nozzle)
When putting the pipette tips on the pipette gun, many people will knock on the pipette box a few times, which is the wrong way, because it will cause the internal accessories of the pipette gun (such as springs). The instantaneous impact force produced by the knocking becomes loose and may even cause the scale adjustment knob to become stuck. The correct method is to insert the pipette (instrument) vertically into the tip of the pipette, and turn it slightly tightly with a little force to make it tightly combined. If it is a multi-channel (such as 8 or 12) pipette gun, you can align the first pipette pipette with the first pipette tip, and then insert it obliquely, shake it forward and backward to lock it. The sign that the gun head is clamped is slightly over the O-ring, and you can see that the connection part forms a clear seal ring.
Pipetting method
Before pipetting, make sure that the pipette, tip and liquid are at the same temperature. When aspirating liquid, the pipette is kept upright and the tip of the pipette is inserted 2-3 mm below the liquid surface. Before sucking liquid, you can suck the liquid several times to wet the suction nozzle (especially when you want to suck a viscous liquid or a liquid with a different density). At this time, two pipetting methods can be used.
One is the forward pipetting method. Use your thumb to press the button to the first stop, and then slowly release the button to return to the original point. Then press the button to the first stop point to discharge the liquid, and after a while, continue to press the button to the second stop point to blow out the remaining liquid. Finally release the button.
The second is the reverse pipetting method. This method is generally used to transfer high-viscosity liquids, biologically active liquids, easy-foaming liquids, or very small amounts of liquids. The principle is to inhale more than the set range of liquid first, and do not need to blow out residual liquid when transferring liquid. First press the button to the second stop, and slowly release the button to the origin. Then press the button to the first stop point to discharge the liquid with the set range. Continue to hold the button at the first stop point (do not press down again), remove the tip of the residual liquid, and discard it.
Correct placement of the pipette
After use, you can hang it vertically on the pipette holder, but be careful not to drop it. When there is liquid in the pipette tip, do not place the pipette horizontally or upside down to prevent the backflow of liquid from corroding the piston spring.
Notes on the maintenance of the pipette (pipette gun)
If not in use, adjust the range of the pipette to the maximum scale, and leave the spring in a relaxed state to protect the spring.
It is best to clean the pipette regularly, you can use soapy water or 60% isopropyl alcohol, then use distilled water to clean, dry naturally.
Before high temperature disinfection, make sure that the pipette can adapt to high temperature.
Calibration can be carried out in a 20-25 degree environment by repeating the method of weighing distilled water several times.
When using it, check for liquid leakage. In the method, after absorbing the liquid, hang it vertically and let it stand for a few seconds to see if the liquid level drops. If the liquid leaks, the reasons are roughly
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