Brief description of the principle of gradient elution

Gradient elution is also known as gradient elution or program elution. In gas chromatography, a temperature-programmed method is widely used in order to improve the separation and shrinkage analysis period for a wide boiling range sample. Gradient elution is used in liquid chromatography. In the same analysis cycle, the concentration ratio of the mobile phase is continuously changed to a certain extent, which is called gradient elution. Therefore, components with a large difference in properties in a complex sample can be separated for a suitable separation factor k.

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