Two methods of pipetting

Before pipetting, make sure that the pipette, tip and liquid are at the same temperature. When aspirating the liquid, keep the pipette upright and insert the tip of the pipette 2-3 mm below the liquid surface. Before sucking liquid, you can suck the liquid several times to wet the suction nozzle (especially when you want to suck a viscous liquid or a liquid with a different density). At this time, two pipetting methods can be used.

One is the forward pipetting method. Use your thumb to press the button to the first stop, and then slowly release the button to return to the original point. Then press the button to the first stop point to discharge the liquid, and after a while, continue to press the button to the second stop point to blow out the remaining liquid. Finally release the button.

The second is the reverse pipetting method. This method is generally used to transfer high-viscosity liquids, biologically active liquids, easy-foaming liquids, or very small amounts of liquids. The principle is to inhale more than the set range of liquid first, and do not need to blow out residual liquid when transferring liquid. First press the button to the second stop, and slowly release the button to the origin. Then press the button to the first stop point to discharge the liquid with the set range. Continue to hold the button at the first stop point (do not press down again), remove the tip of the residual liquid, and discard it.

Correct placement of the pipette

After use, you can hang it vertically on the pipette holder, but be careful not to drop it. When there is liquid in the pipette tip, do not place the pipette horizontally or upside down to prevent the backflow of liquid from corroding the piston spring.