Alkaline pectinase (EC: 4.2.2.2) is a type of enzyme that breaks the pectin backbone by trans-elimination under alkaline conditions to produce unsaturated oligogalacturonic acid. Hemp degumming, cotton fabric refining and other fields have a wide range of applications. Compared with the traditional high-alkali and high-temperature treatment methods, the use of alkaline pectinase not only has a good removal effect on pectin, but also has less damage to natural cellulose fibers, and can reduce material consumption and environmental burden. Industry has opened a green and clean road.
The Research Group of Structural Bioinformatics and Integrated Systems Biology of Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences has carried out a series of work on how to achieve the key link of industrialized high yield of pectate lyase. In this work, a degenerate PCR method was used to mine a new pectin lyase sequence from the environmental genome and express it in E. coli. Through the study of its enzymatic properties and the evaluation of hemp degumming effect, it is found that the enzyme has great practical application potential. This work further uses a high cell density fermentation optimization method to achieve high yields of pectin lyase in E. coli. The output on a 7L fermentation tank can reach 1816.2 U / mL, which is much higher than the level reported by peers, which is beneficial to industrialization The application of production has improved our research level in key technical fields such as fermentation production of important industrial enzymes and cleaner production in the textile industry.
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