Technical progress and application of gel imaging system

Technology Development and Application of Gel Imaging System East China Biological Equipment Net / 2011-11-07

With the gradual popularization of molecular biology research, the demand for gel imaging systems in China is growing

Regardless of the purpose, the components of the gel imaging system are similar. Each has a shooting system, a dark box with a special light source, and software for acquiring and analyzing gel pictures. "However, most gel imaging systems provide different product characteristics to meet the needs of different scientific research. The rapid development of electronic technology, optical technology and imaging analysis software makes the imaging operation more humanized.

The domestic gel imaging system is mainly produced by Shanghai Jiapeng Technology Co., Ltd. ZF series gel imaging system, the cost performance is very high.

At present, the main products imported are Bio-Rad's products. The ChemiDoc XRS system is designed for high-resolution chemiluminescence and fluorescence imaging applications. The system's features include: a 1.3-megapixel super-cooled CCD camera, a tightly opaque darkroom, a sliding transmissometer, and a software-controlled zoom lens that focuses on zoom, focus, aperture, real-time imaging, and dynamic flat field processing. Another more basic use model, Gel Doc XR, is mainly used for fast high-resolution imaging, but there is no chemiluminescence imaging function. The system includes a darkroom, a 1.4-megapixel CCD camera, UV and white light illumination, amber filter glass, and UV protective cover. The Gel Doc XR system can also be upgraded to the ChemiDoc XRS system. Both systems include image acquisition and analysis software-Quantity One. Bio-Rad also offers two models of gel imaging systems with higher resolution and better sensitivity: VersaDoc Model 4000 and VersaDoc Model 5000. The VersaDoc Model 4000 system has a 3.2 megapixel CCD camera that provides the best resolution. The system is particularly suitable for proteome analysis. For example, you can use the Quantity One 1-D analysis software to estimate the molecular weight and number of protein samples, and you can also use the PDQuest 2-D analysis software to analyze differences in protein expression products.

The well-known Alpha Innotech company also provides a wide range of products for scientific research and budget requirements, such as the current relatively new and high-end products-FluorChem SP, which is a kind of chemiluminescence, fluorescence, and Products for visible light applications. High resolution (science-grade CCD), 4 megapixels, low signal-to-noise ratio, low dark current (dark current), absolute and adjustable cooling temperature (for higher-end systems) Alpha Innotech's AlphaEaseFC software, its unique algorithm improves imaging resolution through technologies such as three-dimensional image contour imaging, image sharpening, and signal-to-noise reduction. AlphaEaseFC software provides easy-to-use one-click to complete 1D lane analysis, 2D spot density, MW / Rf (molecular weight / mobility) calculation, cloning and cell counting, microtiter plate analysis, chip analysis, distance measurement and gel Scoring and other functions.

Alpha Innotech also provides users with an affordable AlphaDigiDocRT 2 product, which is a real-time gel imaging system that can be used as long as it has a computer (connected via USB) and a UV transmissometer. In addition, Kodak ’s molecular imaging system stated to its senior marketing specialist Allison Sova: “Kodak ’s Gel Logic ’s family of gel imaging systems allows researchers to choose the performance they need today, and it can be economical in the future. A simple upgrade system to meet future needs. In short, Kodak provides researchers with greater flexibility to meet their laboratory's ever-increasing imaging capabilities. It is also a system that can be upgraded.

Kodak's Gel Logic 100 is Kodak's basic imaging system. It contains a 1 megapixel digital CCD camera, a Gel Logic imaging operating room, and an ethidium bromide bandpass filter. And the award-winning Molecular Imaging software from Kodak provides features not found in other imaging systems. The software can be used in Mac operating systems or Windows operating systems.

Gel Logic 2200 digital imaging system is Kodak's top gel imaging system. Gel Logic 2200 uses a 2.2 megapixel cooled CCD camera, suitable for higher sensitivity chemiluminescence and low-brightness fluorescence detection. The system also integrates white and ultraviolet light sources. Kodak claims that the system can detect picomolar to femtomolar levels of fluorescence signals and achieve the same sensitivity as autoradiographic film.

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